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The nurse didn't shake the vaccine before use

Hi. About a week ago I got my second HPV shot (Gardasil). But I assume the nurse didn't wash her hands before the injection nor she shook the vaccine before the injection. Does this mean my shot will be be less effective and what should I do? (the vaccine was in my bag and I went few floors up for the injection so there was little unwanted shakes in my bag few minutes before the injection) thank you :)
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