I don't know the answer to your question, so I'm answering to bring your question back to the top.
Someone will reply that knows the answer.
Friend999
Takeing your Sub properly is important for full absorption. Drinking something hot right before is suggested. Keeping it under your tongue until completely disolved (5-20 minutes), no talking, smoking or drinking while you're doing your dose.
I don't think spliting your dose has any effect on max effect, but alot of people do split it so that the effect is more balanced thruout the day.