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"Phantom" Bacterias?

I had skin biopsy. Microscope examination shows supparative folliculitis, ruptured, a clear indicative of infection. So they did the gram-staining test to see if it is gram-negative bacteria, and if so, what kind it is because it responded to Rocephin IV.

The result was no staining so it is neither negative or positive gram. I am waiting to hear from my dermatologist if they are doing more further test to identify the organism.  

I am frustrated by this...My sinus which also clear up along with skin lesions during Rocephin IV is now starting to give me trouble. A new--my lung is starting to feel funny (they found apical scarring during CT/Pet scan this past April).

So much for 400mg Doxy daily--I am slipping backward with all other symptoms, and are having recurring low grade fever again. I am having bad UTI-like symptoms, my LLMD order urine test to see what turns up--I am afraid it'll come up empty just as it did in the past.  

It is seems I am having "phantom" bacterias swimming around in my body. I doubt borrelia is the sole cause, it has accomplices.

It would be nice to have documented evidence for the future MDs(when I relocate), and also to allow my doctors better tailor the treatment by testing it for susceptibility, instead of trail and error with various antibiotics. My LLMD say we may have to do just that--to find the best combination for me.    

I am having a hard time finding complete list of "non-gram" bacterias, the kind of bacteria that does not staining when using gram stain method or are difficult to gram staining. My head is not in the mood for intensive online research. I could use little help.

Anyone here a microbiologist? If not in this forum, where can I find one to ask for the list of non-gram stained bacterias online?

Thanks
Best Answer
Avatar universal
Borrelia is neither gram negative nor gram positive, but in the past, it was mistakenly thought to be gram negaive.  Some old publications out there still call it gram negative (incorrectly). Here is a good biological description.

http://textbookofbacteriology.net/Lyme.html

Bartonella has been known to cause skin lesions.  Have you been tested at IGeneX for it?  You might want to get tested for both b.henselae and b.quintana (I don't know who offers tests b.quintana.) Both are present and common in the Italy/Greece part of the world. (I seem to remember you mentioned being there.)

Why doesn't your LLMD just put you back on Rocephin?  Didn't you say your insurance company already approved a year's worth?  And doctors in New York shouldn't have to worry about the state medical board coming after them anymore. There was some kind of political deal in New York where Lyme patient advocates agreed to stop pressing for a physician protection bill (like California's) if the state medical board would leave Lyme treating doctors alone.

Sorry to hear you are regressing.  I am, too. I have been off abx 6 weeks.  I do not like going backwards and I am anxious to get back on abx.
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Avatar universal
:D. I make plenty of iPad swipe mistakes, too!

The LymeMD writes in his blog about seeing Bartonella like organisms on a smear or wet mount. This link should work.  There is a search box in the upper left corner where you can type in words, and it will return every post with those words.

http://lymemd.blogspot.com/search?q=Bartonella+organisms+stain

You could certainly try the IGeneX tests for Bartonella.  I tested positive only on the antibody test.  Actually, I was "NOT NEGATIVE." I was exactly halfway in between positive and negative.  Considering I probably got it six years earlier, I was just happy that any antibodies showed up.  The LLMD called it a positive.

There are some other bacteria that are neither gram negative nor gram positive. Tuberculosis is one. You can Google "neither Gram positive or gram negative" and troll through the results.

The political deal with the medical board in NY is described in Pamela Weintraub's book, "Cure Unknown." It was published in 2008.
Helpful - 0
1763947 tn?1334055319
When you see the LLMD, ask him/her to do an IgenX co-infection test. Besides lesions in stomac, vagina and brain, I now have them in my ears.
Bartonella causes lesions and IgenX should find it.
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Avatar universal
Idoit me---I didn't think of enter in key words in search engine---of course--excuse my "lyme brain"

Within few minutes, I found these:

"B henselae is a gram-negative bacterium, but this organism does not readily stain with a Gram stain."

"The Rickettsia, Ehrlichia, Anaplasma and Coxiella are all small obligate intracellular parasites which were once thought to be part of the same family. Now, however, they are considered to be distinct unrelated bacteria. Like the Chlamydia these bacteria were once thought to be viruses because of their small size and intracellular life cycle. However, they are true bacteria, structurally similar to Gram negative bacteria. They are small Gram negative coccobacilli that are normally stained with Giemsa since they stain poorly with the Gram stain. "

Even better--complete chart of bacterias.

http://pmj.bmj.com/content/77/905/148/F1.large.jpg

Now, only if I could give myself a star by select myself as Best Answer! Lol.
Helpful - 0
Avatar universal
Oops....swipe ipad screen--another star to you!  I am in my bed, unable to sleep, with heat pad wrapping around my head.

So borrelia is the only bacteria is neither gram negative or gram positive?

I am trying to see my dermatalogist tomorrow-asking her to  look for such bacterias. She is very close/friend with pathologist who exams my skin biospy, and actually texting him in middle of my visit to him.  I think they both have interests in my bizarre case.

I wonder if the pathologist office have dark field microscope.

Would be nice to have concerte evidence in my hand to shove into my neurologist's face.  I saw my neurologist last week--says my herx reactions confirms the diagnosis of lyme.  

He say 4 weeks of rocephin is more than enough, and was upset I even asked for more.  So it is over with this neurologist.

I will see my LLMD in two weeks. Actually my insurance approved ten years worth, not a year worth.

I am not aware of political deal--I'll check.

ID specialist did test me for bartonella (blood serum), it was negative-for both heneselae and quintana. It wasn't test at Igenex lab.  Umm...let me check online to see if bartonella can be gram stained...Be back..
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